This course provides a rigorous introduction to the theory underlying widely used biophysical methods, which will be illustrated by practical applications to contemporary biomedical research problems. The course has two equally important goals. The first goal is for students to understand the fundamental approaches used by physical chemists to understand the behavior of molecules and develop related analytical tools. The second goal is to prepare students to apply these methods themselves to their own research projects. The course will be divided into eight modules: (i) solution thermodynamics; (ii) statistical analysis of experimental data; (iii) hydrodynamics; (iv) nuclear magnetic resonance spectroscopy; (v) optical spectroscopy with an emphasis on fluorescence; (vi) diffraction & cryo-EM methods; and (vii) molecular dynamics simulation methods. In each module, the underlying physical theories and models will be presented and used to derive the mathematical equations applied to the analysis of experimental data. Weekly recitations will emphasize the analysis of real experimental data and understanding the applications of biophysical experimentation in published research papers.  The first three modules will be covered in Biophysical Chemistry I during the fall term, while the next three will be covered in Biophysical Chemistry II (BIOC GU4324) during the spring term.  Material related to the final module (molecular dynamics simulations) will be divided between the two terms.