Proteomics Laboratory
Fall 2022
Section Number: 
Days of the Week: 
12:30pm - 3:30pm
743 Mudd
Number of Credits: 
Course Description: 

This course deals with the proteome: the expressed protein complement of a cell, organelle, matrix, tissue, organ or organism. The study of the proteome (proteomics) is broadly applicable to life sciences research, and is increasingly important in academic, government and industrial research through extension of the impact of advances in genomics. These techniques are being applied to basic research, exploratory studies of cancer and other diseases, drug discovery and many other topics. Emphasis will be on mastery of practical techniques of sample preparation, liquid chromatography/ mass spectrometry (LC/MS) with electrospray ionization, and Matrix-Assisted Laser Desorption and Ionization (MALDI-TOF) mass spectrometry. Database searching and interpretation for identification of proteins will be intensively studied, and practiced supported by background tutorials and exercises covering other techniques used in proteomics. Open to students in M.A. in Biotechnology Program (points can be counted against laboratory requirement for that program), Ph.D. and advanced undergraduate students with background in genetics or molecular biology. Students should be comfortable with basic biotechnology laboratory techniques as well as being interested in doing computational work in a Windows environment.

Business Office

Department of Biological Sciences
500 Fairchild Center
Mail Code 2401
Columbia University
1212 Amsterdam Avenue
New York, NY 10027

Academic Office

Department of Biological Sciences
600 Fairchild Center
Mail Code 2402
Columbia University
1212 Amsterdam Avenue
New York, NY 10027
[email protected]
212 854-4581