Host: John Hunt
Title: Translation affects mRNA stability in higher organisms
Abstract: Messenger RNA (mRNA) degradation and mRNA translation represent two fundamental steps in the regulation of gene expression. Stability of the mRNA affects mRNA levels which in turn, impact protein production. Alterations in mRNA degradation leads to developmental defects and human disease. Likewise, aberrant mRNA translation has been implicated in protein misfolding and neurodegenerative disease, viral infection and developmental defects. We are interested in discovering and understanding how post-transcriptional regulatory pathways works in higher organism.
Ribosomes are the most highly RNA-binding in the cell, and while its main function is to decodes nucleotide into amino acid sequences. Translation can also affect mRNA stability depending on codon composition. This regulatory event is different from codon usage or bias and is known as codon optimality, defined as the property of given codons to regulate mRNA stability in a translation dependent manner. The regulatory strength of this codon-mediated mechanism is remarkable. Combining genome-wide and classical molecular approaches, as well as human cells and zebrafish embryos, we want to elucidate how translation affects mRNA stability, dissect the molecular mechanism, define the relation with other post-transcriptional pathways (microRNA and m6A) and discover novel post-transcriptional mechanisms affecting mRNA stability and translation.