Seminar - Kurt Fredrick

photo of Dr. Kurt Fredrick
January 29, 2020 - 12:00pm
Location: 
601 Fairchild

Department of Microbiology

The Ohio State University

Hosts: John Hunt, Ruben Gonzalez

Title: Kozak-like sequences help tune translation in bacteria

Abstract: In all cells, initiation of translation is tuned by intrinsic features of the mRNA. Here, we analyze translation in F. johnsoniae, a representative of the Bacteroidetes. Members of this phylum naturally lack Shine-Dalgarno (SD) sequences in their mRNA, and yet their ribosomes retain the conserved anti-SD sequence. Translation initiation is tuned by mRNA secondary structure and by the identities of several key nucleotides upstream of the start codon. Positive determinants include adenine at position -3, reminiscent of the Kozak sequence of Eukarya. Comparative analysis of E. coli reveals use of the same Kozak-like sequence to enhance initiation, suggesting an ancient and widespread mechanism. Elimination of contacts between A-3 and the conserved beta-hairpin of ribosomal protein uS7 fails to diminish the contribution of A-3 to initiation, suggesting an indirect mode of recognition. Also, we find that, in the Bacteroidetes, the trinucleotide AUG is underrepresented in the vicinity of the start codon, which presumably helps compensate for the absence of SD sequences in these organisms.

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